Techniques

A quick overview of the techniques used in this project

RNA isolation

Isolated RNA from hybridoma cell lines grown in the Cornelissen lab.

Isolated RNA was used as a template to create cDNA using PCR

Structure of DNA. by Sangharsh Lohakare

cDNA fragments were added to a vector and grown on agar plates in a TOPO cloning reaction

Scientist using a petri dish. "Science" by Wix.com

PCR amplification was used to rapidly produce multiple copies of the target DNA

Colonies grown on agar. "Serratia marcescens colonies on BTB agar medium. Produces red pigment." by Masakazu Sasaki